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  • Reduced expression of the vca0421 gene of Vibrio cholerae O1 results in innate resistance to ciprofloxacin.

Reduced expression of the vca0421 gene of Vibrio cholerae O1 results in innate resistance to ciprofloxacin.

Antimicrobial agents and chemotherapy (2010-09-02)
Jun Okuda, Naoki Hayashi, Yuka Wakahara, Naomasa Gotoh
ABSTRACT

A mini-Tn5 insertion into a ciprofloxacin (CIP)-resistant mutant of Vibrio cholerae O1 revealed that overexpression of the vca0421 gene, which encodes a hypothetical protein, in the CIP-resistant mutant carrying a mutation in the quinolone resistance-determining region (QRDR) of the gyrA gene causes sensitization to CIP. We propose a new intrinsic mechanism of resistance to fluoroquinolones due to the inherently reduced expression of the vca0421 gene in V. cholerae O1.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tetracycline, 98.0-102.0% (HPLC)
Supelco
Norfloxacin, analytical standard, ≥98% (TLC)
Sigma-Aldrich
Sparfloxacin, ≥98.0% (HPLC)
Sigma-Aldrich
Ciprofloxacin, ≥98% (HPLC)
Supelco
Ciprofloxacin, VETRANAL®, analytical standard
Supelco
Norfloxacin, VETRANAL®, analytical standard