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  • Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting.

Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting.

Science advances (2021-11-25)
Xukun Lu, Yu Zhang, Lijuan Wang, Leyun Wang, Huili Wang, Qianhua Xu, Yunlong Xiang, Chaolei Chen, Feng Kong, Weikun Xia, Zili Lin, Sinan Ma, Ling Liu, Xiangguo Wang, Hemin Ni, Wei Li, Yong Guo, Wei Xie
ABSTRACT

[Figure: see text].

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Protease from Streptomyces griseus, powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
Sigma-Aldrich
Tyrode′s Solution, Acidic, liquid, sterile-filtered, suitable for mouse embryo cell culture
Sigma-Aldrich
M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture
Sigma-Aldrich
ESGRO® Recombinant Mouse LIF Protein, ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.
Sigma-Aldrich
MG-132(R), ≥95% (HPLC)