Merck
  • Home
  • Search Results
  • A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids.

A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids.

Nature protocols (2016-03-18)
Tejia Zhang, Shili Chen, Ismail Syed, Marcus Ståhlman, Matthew J Kolar, Edwin A Homan, Qian Chu, Ulf Smith, Jan Borén, Barbara B Kahn, Alan Saghatelian
ABSTRACT

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous mammalian lipids with antidiabetic and anti-inflammatory effects. We previously identified 16 different FAHFA families, such as branched palmitic acid esters of hydroxy stearic acids (PAHSAs); each family consists of multiple isomers in which the branched ester is at different positions (e.g., 5- and 9-PAHSA). We anticipate increased need for PAHSA measurements as markers of metabolic and inflammatory health. In this protocol, we provide a detailed description of the extraction of FAHFAs from human or mouse tissues, their enrichment by solid-phase extraction and subsequent analysis by LC-MS. For a sample size of 6-12, the time frame is 2-3 d.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium citrate tribasic dihydrate, for molecular biology, ≥99%
Sigma-Aldrich
Ammonium hydroxide solution, 28% NH3 in H2O, ≥99.99% trace metals basis