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A fluorimetric and enzymatic method for the estimation of serum total bile acids.

Journal of clinical pathology (1970-10-01)
G M Murphy, B H Billing, D N Baron
ABSTRACT

The enzymatic technique using 3alpha-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced patients. The range of normal values in serum was found to be 0-4.7 mumol/litre for males and 1.0-8.2 mumol/litre for females.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium glycocholate hydrate, ≥95% (TLC)
Sigma-Aldrich
Hydrazine hydrate solution, puriss. p.a., 24-26% in H2O (RT)
Sigma-Aldrich
Hydrazine hydrate, reagent grade, N2H4 50-60 %