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Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites.

Marine drugs (2012-12-29)
Anahit Penesyan, Francesco Ballestriero, Malak Daim, Staffan Kjelleberg, Torsten Thomas, Suhelen Egan
ABSTRACT

A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the "hit-rate" and unmasking multiple activities from the same bacterial source.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
REDTaq® ReadyMix PCR Reaction Mix, Complete PCR reagent with standard Taq DNA Polymerase and inert dye
Sigma-Aldrich
10× REDTaq® PCR Reaction Buffer, To be used with REDTaq® DNA Polymerase