Merck

A comparative study of polyelectrolyte-dye interactions.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2009-10-17)
R Nandini, B Vishalakshi
ABSTRACT

The interaction of Azure B with sodium alginate and heparin in aqueous solution has been studied by spectrophotometric method. Absorbance of Azure B at 645 nm decreases and a new band appeared at 545 nm and at 556 nm respectively which indicated that a new metachromatic complex formed. A linear decrease in absorbance is noted. It was found that sodium alginate is more effective than heparin in decreasing the absorbance of Azure B at 645 nm. The stoichiometry of sodium alginate or heparin with Azure B was determined by spectrophotometry. The results suggested that the interaction between Azure B with sodium alginate or heparin was a result of electrostatic forces and the difference between heparin and sodium alginate were attributed to the different negative charge number on repetitive disaccharides unit. Studies on the effect of alcohol or urea indicated that sodium alginate and heparin interacted with the aggregates of Azure B. Thermodynamic parameters of interaction has been evaluated to determine the stability of the metachromatic complex. The effect of surfactants on reversal of metachromasy has also been studied.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Azure B, prepared by direct synthesis
Sigma-Aldrich
Azure B, certified by the Biological Stain Commission