• Home
  • Search Results
  • Histopathological characteristics of luteal hypertrophy induced by ethylene glycol monomethyl ether with a comparison to normal luteal morphology in rats.

Histopathological characteristics of luteal hypertrophy induced by ethylene glycol monomethyl ether with a comparison to normal luteal morphology in rats.

Toxicologic pathology (2011-03-23)
Yoshikazu Taketa, Akira Inomata, Satoru Hosokawa, Jiro Sonoda, Kazuhiro Hayakawa, Kyoko Nakano, Yuki Momozawa, Joji Yamate, Midori Yoshida, Toyohiko Aoki, Kazuo Tsukidate
ABSTRACT

Ethylene glycol monomethyl ether (EGME) is a known reproductive toxicant that induces luteal hypertrophy in rat ovaries. In this study, we characterized the histopathological features of corpora lutea (CL) from EGME-treated rats and compared them with normal CL formation and regression. Normally cycling female Sprague-Dawley rats were treated with 5-bromo-2'-deoxyuridine (BrdU) intraperitoneally on the morning of estrus and their ovaries were examined 1 (metestrus), 4 (estrus), 8 (estrus), or 12 (estrus) days later to observe the transition of BrdU-labeled cells within in the CL. CL at each time point of estrus stage were classified into 4 types: Type I (newly formed CL), Type II (mature CL), Type III (regressing CL), and Type IV (residual CL). CL almost fully regressed within 4 estrus cycles. In contrast, in female rats given EGME orally (30, 100, or 300 mg/kg for 2 or 4 weeks), luteal cells were hypertrophic with abundant cytoplasm. Although the size of CL varied, all CL in EGME-treated rats had histological features similar to Type II CL, but they were more hypertrophic with less apoptosis. These results suggest that EGME has a luteal hypertrophic effect on all CL phases, including regression.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Methoxyethanol, anhydrous, 99.8%
Sigma-Aldrich
2-Methoxyethanol, suitable for HPLC, ≥99.9%
Sigma-Aldrich
2-Methoxyethanol, ReagentPlus®, ≥99.0%, contains 50 ppm BHT as stabilizer
Supelco
2-Methoxyethanol, analytical standard
Sigma-Aldrich
2-Methoxyethanol, contains 50 ppm BHT as stabilizer, ACS reagent, ≥99.3%