• Home
  • Search Results
  • Nitrate and ammonium lead to distinct global dynamic phosphorylation patterns when resupplied to nitrogen-starved Arabidopsis seedlings.

Nitrate and ammonium lead to distinct global dynamic phosphorylation patterns when resupplied to nitrogen-starved Arabidopsis seedlings.

The Plant journal : for cell and molecular biology (2011-11-09)
Wolfgang R Engelsberger, Waltraud X Schulze
ABSTRACT

Nitrogen is an essential macronutrient for plant growth and development. Inorganic nitrogen and its assimilation products control various metabolic, physiological and developmental processes. Although the transcriptional responses induced by nitrogen have been extensively studied in the past, our work here focused on the discovery of candidate proteins for regulatory events that are complementary to transcriptional changes. Most signaling pathways involve modulation of protein abundance and/or activity by protein phosphorylation. Therefore, we analyzed the dynamic changes in protein phosphorylation in membrane and soluble proteins from plants exposed to rapid changes in nutrient availability over a time course of 30 min. Plants were starved of nitrogen and subsequently resupplied with nitrogen in the form of nitrate or ammonium. Proteins with maximum change in their phosphorylation level at up to 5 min after nitrogen resupply (fast responses) included GPI-anchored proteins, receptor kinases and transcription factors, while proteins with maximum change in their phosphorylation level after 10 min of nitrogen resupply (late responses) included proteins involved in protein synthesis and degradation, as well as proteins with functions in central metabolism and hormone metabolism. Resupply of nitrogen in the form of nitrate or ammonium resulted in distinct phosphorylation patterns, mainly of proteins with signaling functions, transcription factors and transporters.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium nitrate, ACS reagent, ≥99.0%
Sigma-Aldrich
Potassium nitrate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99%
Sigma-Aldrich
Potassium nitrate, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Potassium nitrate, BioReagent, suitable for cell culture, suitable for plant cell culture
Supelco
Mettler-Toledo Calibration substance ME 51143095, Potassium nitrate, traceable to primary standards (LGC)
Supelco
Nitrate ion standard solution, 0.01 M NO3-, for ion-selective electrodes
Sigma-Aldrich
Potassium nitrate, 99.999% trace metals basis
Sigma-Aldrich
Potassium nitrate, 99.99% trace metals basis
Sigma-Aldrich
Potassium nitrate, BioXtra, ≥99.0%
Sigma-Aldrich
Potassium nitrate, puriss., meets analytical specification of Ph Eur, BP, USP, FCC, E252, 99.0-100.5%