Quercetin as a shuttle for labile iron.

Journal of inorganic biochemistry (2011-12-17)
Mayara Marinovic Baccan, Orlando Chiarelli-Neto, Regina Mara Silva Pereira, Breno Pannia Espósito
ABSTRACT

The antioxidant activity of flavonoids may involve their ability to complex body iron in non-redox-active forms. In this study, it was found that the catechol flavonoids rutin and quercetin are able to suppress redox-active labile plasma iron (LPI) in both buffered solution and in iron-overloaded sera. Both flavonoids are effective in loading the metal into the iron-transport protein transferrin. Iron derivatives of quercetin and rutin are able to permeate cell membranes, however, only free quercetin is able to gain access to the cytosol and decrease intracellular labile iron pools. These results suggest that the antioxidant activity of quercetin may be dependent on its ability to shuttle labile iron from cell compartments followed by its transfer to transferrin.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ammonium iron(II) sulfate hexahydrate, ACS reagent, 99%
Sigma-Aldrich
Ammonium iron(II) sulfate hexahydrate, 99.997% trace metals basis
Sigma-Aldrich
Ammonium iron(II) sulfate hexahydrate, ReagentPlus®, ≥98%
Sigma-Aldrich
Ammonium iron(II) sulfate hexahydrate, BioUltra, ≥99.0% (RT)
Sigma-Aldrich
Ammonium iron(II) sulfate hexahydrate, BioXtra, ≥98%