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Digestive stability of hydroxytyrosol, hydroxytyrosyl acetate and alkyl hydroxytyrosyl ethers.

International journal of food sciences and nutrition (2012-01-25)
Gema Pereira-Caro, Beatriz Sarriá, Andrés Madrona, José Luis Espartero, María E Escuderos, Laura Bravo, Raquel Mateos
ABSTRACT

The digestive stability of two natural antioxidant compounds present in virgin olive oil, hydroxytyrosol (HTy) and hydroxytyrosyl acetate (HTy-Ac) and a new series of hydroxytyrosyl ethers (methyl, ethyl and butyl hydroxytyrosyl ethers) was evaluated by a simulated digestion procedure. High recovery of all compounds after gastric digestion was obtained, although they showed a statistically significant lower stability after pancreatic-bile salts digestion. HTy-Ac was partially hydrolyzed into free HTy, whereas after intestinal digestion, HTy was converted into 3,4-dihydroxyphenyl acetic acid (DOPAC), and HTy-Ac was hydrolyzed to HTy and subsequently transformed into DOPAC. In contrast, no chemical modification of hydroxytyrosyl ethers during in vitro digestion was observed. In summary, HTy, HTy-Ac and hydroxytyrosyl ethers show high and interesting digestive stability and the new synthetic hydroxytyrosyl ethers showed enhanced chemical stability compared to HTy and HTy-Ac.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pancreatin from porcine pancreas, ≥3 × USP specifications
Sigma-Aldrich
Pancreatin from porcine pancreas, 8 × USP specifications
Sigma-Aldrich
Pancreatin from porcine pancreas, 4 × USP specifications
Sigma-Aldrich
Pancreatin from porcine pancreas, powder, suitable for cell culture, 4 × USP specifications
Sigma-Aldrich
3-Hydroxytyrosol, ≥98% (HPLC)
Sigma-Aldrich
Pancreatin from porcine pancreas, Vetec, reagent grade