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  • A comparison of the contrast stains, Chicago blue, chlorazole black, and Parker ink, for the rapid diagnosis of skin and nail infections.

A comparison of the contrast stains, Chicago blue, chlorazole black, and Parker ink, for the rapid diagnosis of skin and nail infections.

International journal of dermatology (2012-07-14)
Evanthia Tambosis, Christopher Lim
ABSTRACT

The cheapest, most rapid method for the diagnosis of mycotic skin and nail infections is direct examination of samples using a KOH preparation. The standard KOH wet mount lacks a color contrast. Various contrast dyes are available. Parker ink is one which has been extensively used, however in recent times the stain no longer provides a suitable color contrast. Two other dyes that are available are Chicago blue and chlorazole black. 100 samples of skin or nails were prospectively examined using Chicago blue, chlorazole black, and Parker ink-KOH stains. The samples were also cultured, with culture being the diagnostic gold standard. The sensitivities and specificities of the Chicago blue, chlorazole black and Parker ink stains were 78% & 96%; 63% & 97%; and 48% and 96%, respectively. The Chicago blue-KOH stain provides a good color contrast and is a suitable replacement for the Parker ink-KOH stain. It is suitable for use in the dermatologist's office or in the laboratory.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium hydroxide, ACS reagent, ≥85%, pellets
Sigma-Aldrich
Potassium hydroxide, reagent grade, 90%, flakes
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Potassium hydroxide solution, 45 wt. % in H2O
Sigma-Aldrich
Potassium hydroxide, semiconductor grade, pellets, 99.99% trace metals basis (Purity excludes sodium content.)
Supelco
Potassium hydroxide solution, volumetric, 8.0 M KOH (8.0N)
Sigma-Aldrich
Potassium hydroxide, BioXtra, ≥85% KOH basis
Sigma-Aldrich
Potassium hydroxide, ≥85% KOH basis, pellets, white
Supelco
Potassium hydroxide concentrate, 0.1 M KOH in water (0.1N), Eluent concentrate for IC
Sigma-Aldrich
Potassium hydroxide, pellets, reag. Ph. Eur., ≥85%
Sigma-Aldrich
Potassium hydroxide, technical, ≥85%, powder
Sigma-Aldrich
Chlorazol Black
Sigma-Aldrich
Chicago Sky Blue 6B, powder
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Potassium hydroxide, puriss., meets analytical specification of Ph. Eur., BP, 85-100.5%, pellets
Sigma-Aldrich
Potassium hydroxide, puriss. p.a., ≥86% (T), pellets
Sigma-Aldrich
Potassium hydroxide, tested according to Ph. Eur.
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Potassium hydroxide, free-flowing, Redi-Dri, ACS reagent, ≥85%, pellets