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Highly luminescent-paramagnetic nanophosphor probes for in vitro high-contrast imaging of human breast cancer cells.

Small (Weinheim an der Bergstrasse, Germany) (2012-07-19)
Bipin Kumar Gupta, Tharangattu N Narayanan, Sajna Antony Vithayathil, Yean Lee, Shyny Koshy, Arava Leela Mohana Reddy, Avishek Saha, V Shanker, V N Singh, Benny Abraham Kaipparettu, Angel A Martí, Pulickel M Ajayan
ABSTRACT

Highly luminescent-paramagnetic nanophosphors have a seminal role in biotechnology and biomedical research due to their potential applications in biolabeling, bioimaging, and drug delivery. Herein, the synthesis of high-quality, ultrafine, europium-doped yttrium oxide nanophosphors (Y(1.9)O(3):Eu(0.1)(3+)) using a modified sol-gel technique is reported and in vitro fluorescence imaging studies are demonstrated in human breast cancer cells. These highly luminescent nanophosphors with an average particle size of ≈6 nm provide high-contrast optical imaging and decreased light scattering. In vitro cellular uptake is shown by fluorescence microscopy, which visualizes the characteristic intense hypersensitive red emission of Eu(3+) peaking at 610 nm ((5)D(0)-(7)F(2)) upon 246 nm UV light excitation. No apparent cytotoxicity is observed. Subsequently, time-resolved emission spectroscopy and SQUID magnetometry measurements demonstrate a photoluminescence decay time in milliseconds and paramagnetic behavior, which assure applications of the nanophosphors in biomedical studies.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Yttrium(III) oxide, 99.99% trace metals basis
Sigma-Aldrich
Yttrium(III) oxide, nanopowder, <50 nm particle size
Sigma-Aldrich
Yttrium(III) oxide, 99.999% trace metals basis
Sigma-Aldrich
Yttrium(III) oxide, sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.99% trace metals basis
Sigma-Aldrich
Yttrium(III) oxide, dispersion, 10 wt. % in isopropanol, nanoparticles, <100 nm (DLS), ≥99.9% trace metals basis