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Myrtus communis L. infusions: the effect of infusion time on phytochemical composition, antioxidant, and antimicrobial activities.

Journal of food science (2012-08-15)
Chokri Messaoud, Abdelmonoem Laabidi, Mohamed Boussaid
ABSTRACT

In traditional medicine, myrtle (Myrtus communis L.) is frequently consumed as an infusion and decoction. In this study, we investigate the phenolic and volatile compositions and antioxidant and antibacterial activities of leaf infusions prepared during 3 different times. The total phenolics contents (146.74 to 179.55 mg GAE/g DM) varied significantly between infusions. Eleven phenolic compounds were identified by reversed-phase high-performance liquid chromatography. Phenolic acids (7.64 to 14.28 μmol/g DM) and flavonol glycosides (7.05 to 12.11 μmol/g DM) were the major phenolic fractions of infusions. Significant quantitative variation in 6 phenolic components was observed between infusions. Sixteen volatile components were identified by gas chromatography (GC) and GC mass spectrometry analyses. The main constituents were 1,8-cineole (42.58% to 51.39%), α-terpineol (9.45% to 9.72%), methyl eugenol (6.69% to 7.11%), and linalool (5.91% to 6.06%). Quantitative variations of the volatile components of the analyzed oils in relation to the infusion time were observed. The antioxidant properties of infusions, assayed through DPPH (2,2- diphenyl-1-picrylhydrazyl) method, β-carotene bleaching test, chelating effect on ferrous ions, and ferric reducing power method, were considerable and varied according to the infusion time. Myrtle infusions exhibited a substantial antimicrobial activity against 6 tested bacteria.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Linalool, 97%
Sigma-Aldrich
Eucalyptol, 99%
Sigma-Aldrich
α-Terpineol, 90%, technical grade
Supelco
Eucalyptol, analytical standard
Supelco
Linalool, analytical standard
Sigma-Aldrich
Methyl eugenol, ≥98%, FCC
Sigma-Aldrich
Linalool, ≥97%, FCC, FG
Sigma-Aldrich
(−)−α-Terpineol, natural, ≥96%, FCC, FG
Sigma-Aldrich
Eucalyptol, natural, ≥99%, FCC, FG
Sigma-Aldrich
Eucalyptol, tested according to Ph. Eur.
Supelco
1,8-Cineole, primary reference standard
Sigma-Aldrich
L-Linalool, natural, ≥95%, FG
Sigma-Aldrich
Terpineol, mixture of isomers, 96%, FG
Sigma-Aldrich
(−)-Linalool, ≥95.0% (sum of enantiomers, GC)
Supelco
Methyl eugenol, analytical standard
Sigma-Aldrich
4-Allyl-1,2-dimethoxybenzene, 99%
Supelco
(−)-Linalool, analytical standard
Supelco
(+)-α-Terpineol, analytical standard
Cineole, European Pharmacopoeia (EP) Reference Standard
Supelco
Eucalyptol, certified reference material, TraceCERT®