Merck

Staining and embedding the whole mouse brain for electron microscopy.

Nature methods (2012-10-23)
Shawn Mikula, Jonas Binding, Winfried Denk
ABSTRACT

The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single-axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block-face electron microscopy.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Osmium tetroxide, Sealed ampule.
Sigma-Aldrich
Os EnCat® 40, extent of labeling: 0.3 mmol/g Os loading
Sigma-Aldrich
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Sigma-Aldrich
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Sigma-Aldrich
Osmium tetroxide solution, suitable for electron microscopy, 4% in H2O
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Osmium tetroxide solution, suitable for electron microscopy, 2% in H2O
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