Study of charge distribution on the surface of biocolloids.

Colloids and surfaces. B, Biointerfaces (2013-01-10)
Ewelina Dziubakiewicz, Katarzyna Hrynkiewicz, Mariusz Walczyk, Bogusław Buszewski
ABSTRACT

Potentiometric titration and zeta potential measurements are crucial techniques for the characterization of the surface properties of bacterial cells. In this study, we investigated the effects of two commonly used electrolytes, NaNO(3) and NaClO(4), on the viability and acid-base properties of Gram-positive bacteria Bacillus subtilis. B. subtilis are non-pathogenic bacteria which are often used to model the surface properties of pathogenic microorganisms of the same genus, including Bacillus anthracis and Bacillus cereus. The survival rates of bacterial cells treated with NaNO(3) were significantly higher in comparison with microorganisms treated with NaClO(4) (5.2-6.8 and 4.1-4.7 log(10) cfu - colony-forming units, respectively). A decrease in the ionic strength (0.1 M, 0.01 M and 0.005 M) of both electrolytes increased viable bacterial cell counts in NaNO(3) treatments and decreased viable bacterial counts in NaClO(4) treatments. Potentiometric titration revealed three dominant types of cell wall functional groups: the carboxyl group (pK(a) values of 4.58-4.89), the amino group (pK(a) values of 9.62-9.89) and the phosphate group (pK(a) values of 7.12-7.49). An increase in the ionic strength of electrolytes led to a decrease in total site concentrations and a drop in buffering capacity at the examined pH values. Based on zeta potential values, measured as a function of pH and ionic strength, the isoelectric point of B. subtilis was determined at pH 2.2 for 0.005 M and 0.01 M NaNO(3). Zeta potential increased with a rise in pH, and it decreased with an increase in ionic strength.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Perchloric acid, ACS reagent, 70%
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