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Determination of captopril by high-performance liquid chromatography with direct electrogenerated chemiluminescence.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2013-01-10)
Yonghua Sun, Zhujun Zhang, Xinfeng Zhang
ABSTRACT

Captopril exhibit electrogenerated chemiluminescence (ECL) in NaNO(3) solution when constant current is exerted. Based on this observation, a direct ECL method coupled with high-performance liquid chromatography (HPLC) separation is developed for determination of captopril in human serum. Factors affected the ECL emission are investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of captopril in the range of 4.0×10(-6)-2.0×10(-3) g mL(-1) and the detection limit is 2×10(-6) g mL(-1) (S/N=3). Compared with the common electrogenerated chemiluminescence experiments, the developed method need no any other fluorescence additives.

MATERIALS
Product Number
Brand
Product Description

Supelco
Captopril, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Captopril, meets USP testing specifications
Sigma-Aldrich
Captopril, ≥98% (HPLC), powder