• Home
  • Search Results
  • Immobilization of Yarrowia lipolytica for aroma production from castor oil.

Immobilization of Yarrowia lipolytica for aroma production from castor oil.

Applied biochemistry and biotechnology (2013-02-20)
Adelaide Braga, Isabel Belo
ABSTRACT

The main aim of this study was to compare different materials for Y. lipolytica immobilization that could be used in the production of γ-decalactone (a peach-like aroma) in order to prevent the toxic effect both of the substrate and the aroma upon the cells. Therefore, cells adsorption onto pieces of methyl polymethacrylate and of DupUM(®) was studied and further used in the biotransformation of castor oil into γ-decalactone. The highest aroma concentration was obtained with immobilized cells in DupUM(®), where reconsumption of the aroma by the cells was prevented, contrarily to what happens with free cells. This is a very promising result for γ-decalactone production, with potential to be used at an industrial level since the use of immobilized cells system will facilitate the conversion of a batch process into a continuous mode keeping high cell density and allowing easier recovery of metabolic products.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Castor oil
Sigma-Aldrich
Castor oil, meets testing specifications per USP
Sigma-Aldrich
γ-Decalactone, ≥98%, FG, FCC
Sigma-Aldrich
Castor oil, tested according to Ph. Eur.
Sigma-Aldrich
γ-Decanolactone, 98%