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  • The identification and characterization of chitotriosidase activity in pancreatin from porcine pancreas.

The identification and characterization of chitotriosidase activity in pancreatin from porcine pancreas.

Molecules (Basel, Switzerland) (2013-03-06)
Chia-Rui Shen, Chao-Lin Liu, Hsiao-Ping Lee, Jeen-Kuan Chen
ABSTRACT

The versatile oligosaccharide biopolymers, chitin and chitosan, are typically produced using enzymatic processes. However, these processes are usually costly because chitinases and chitosanases are available in limited quantities. Fortunately, a number of commercial enzymes can hydrolyze chitin and chitosan to produce long chain chitin or chitosan oligosaccharides. Here, a platform to screen for enzymes with chitinase and chitosanase activities using a single gel with glycol chitin or glycol chitosan as a substrate was applied. SDS-resistant chitinase and chitosanase activities were observed for pancreatin. Its chitotriosidase had an optimal hydrolysis pH of 4 in the substrate specificity assay. This activity was thermally unstable, but independent of 2-mercaptoethanol. This is the first time a chitotriosidase has been identified in the hog. This finding suggests that oligochitosaccharides can be mass-produced inexpensively using pancreatin.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pancreatin from porcine pancreas, ≥3 × USP specifications
Sigma-Aldrich
Pancreatin from porcine pancreas, 8 × USP specifications
Sigma-Aldrich
Pancreatin from porcine pancreas, Vetec, reagent grade
Sigma-Aldrich
Pancreatin from porcine pancreas, 4 × USP specifications
Sigma-Aldrich
Pancreatin from porcine pancreas, powder, suitable for cell culture, 4 × USP specifications
Sigma-Aldrich
Chitin from shrimp shells, practical grade, powder
Sigma-Aldrich
Chitin from shrimp shells, practical grade, coarse flakes
Sigma-Aldrich
Chitin from shrimp shells, BioReagent, suitable for analysis of chitinase, purified powder
Sigma-Aldrich
Chitin from shrimp shells, Vetec, reagent grade