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  • Plant cuticle staining with bismarck brown Y and azure B or toluidine blue O before paraffin extraction.

Plant cuticle staining with bismarck brown Y and azure B or toluidine blue O before paraffin extraction.

Biotechnic & histochemistry : official publication of the Biological Stain Commission (1996-03-01)
E T Graham, P A Joshi
ABSTRACT

Transverse paraffin sections of mature greenwood stems of rose (Rosa x hybrida) and flowering dogwood (Cornus florida L.) were stained with Bismarck brown followed by azure B or toluidine blue 0. The Bismarck brown was replaced by thiazin dye metachromasia in all structures except the cuticle which remained brown or yellow. The interface between the cuticle and exterior cell walls of the epidermis was delineated clearly.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bismarck Brown Y, certified by the Biological Stain Commission, Dye content 50 %
Sigma-Aldrich
Azure B, certified by the Biological Stain Commission
Sigma-Aldrich
Azure B, prepared by direct synthesis