Merck
  • Home
  • Search Results
  • Cerebrospinal fluid levels of thiamine in patients with Alzheimer's disease.

Cerebrospinal fluid levels of thiamine in patients with Alzheimer's disease.

Journal of neural transmission (Vienna, Austria : 1996) (2002-07-12)
J A Molina, F J Jiménez-Jiménez, A Hernánz, E Fernández-Vivancos, S Medina, F de Bustos, C Gómez-Escalonilla, Y Sayed
ABSTRACT

Thiamine is an essential cofactor for several important enzymes involved in brain oxidative metabolism, such as the alpha-ketoglutarate dehydrogenase complex (KGDHC), pyruvate-dehydrogenase complex (PDHC), and transketolase. Some investigators reported decreased thiamine-diphosphate levels and decreased activities of KGDHC, pyruvate-dehydrogenase complex and transketolase in the brain tissue of Alzheimer's disease (AD) patients. We measured cerebrospinal (CSF) levels of thiamine-diphosphate, thiamine-monophosphate, free thiamine, and total thiamine, using ion-pair reversed phase high performance liquid chromatography, in 33 patients with sporadic AD and 32 matched controls. The mean CSF levels of thiamine-derivatives did not differ significantly from those of controls, while the mean plasma levels of thiamine-diphosphate, free and total thiamine were significantly lower in the AD-patient group. CSF and plasma thiamine levels were not correlated with age, age at onset, duration of the disease, and scores of the MiniMental State Examination, with the exception of plasma thiamine-diphosphate with MiniMental State Examination (r = 0.41, p < 0.05) in the AD-patients group. CSF and plasma values did not predict dementia progression, assessed with the MiniMental State Examination scores. These results suggest that CSF thiamine levels are not related with the risk for and the progression of AD.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DL-Homocysteine, ≥95% (titration)
Sigma-Aldrich
3-Methyl-L-histidine
Sigma-Aldrich
L-Isoleucine, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-Isoleucine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
Fumaric acid, BioReagent, suitable for cell culture
Sigma-Aldrich
L-(−)-Arabitol, ≥98%
Sigma-Aldrich
L-Serine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-Serine, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
L-Asparagine, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
L-Asparagine, ≥98% (HPLC)
Sigma-Aldrich
L-2-Aminobutyric acid, ≥99% (titration)
Sigma-Aldrich
L-Phenylalanine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-Phenylalanine, reagent grade, ≥98%
Copper, IRMM®, certified reference material, 0.1 mm foil
SAFC
L-Glutamine
Aluminum, IRMM®, certified reference material, 0.1 mm foil
Sigma-Aldrich
1-Methyl-L-histidine, ≥98.0% (TLC)
Sigma-Aldrich
Zinc, purum, powder
Copper (O), BCR®, certified reference material, rod
Sigma-Aldrich
D-Mannitol, tested according to Ph. Eur.
Copper (S, P), BCR®, certified reference material, chips
Sigma-Aldrich
Fumaric acid, tested according to USP/NF
Sigma-Aldrich
Bismuth, granular, ≥99.99% trace metals basis
Sigma-Aldrich
L-Glutamine, BioUltra, ≥99.5% (NT)
Titanium, IRMM®, certified reference material, 0.1 mm foil
Sigma-Aldrich
L-Serine, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
L-Glutamic acid, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
Zinc, granular, 30-100 mesh, 99%
Copper, IRMM®, certified reference material, 0.5 mm wire
SAFC
L-Isoleucine