Merck

The human serum metabolome.

PloS one (2011-03-02)
Nikolaos Psychogios, David D Hau, Jun Peng, An Chi Guo, Rupasri Mandal, Souhaila Bouatra, Igor Sinelnikov, Ramanarayan Krishnamurthy, Roman Eisner, Bijaya Gautam, Nelson Young, Jianguo Xia, Craig Knox, Edison Dong, Paul Huang, Zsuzsanna Hollander, Theresa L Pedersen, Steven R Smith, Fiona Bamforth, Russ Greiner, Bruce McManus, John W Newman, Theodore Goodfriend, David S Wishart
ABSTRACT

Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic profiling, is leading to increased interest pressures within the metabolomics community to develop centralized metabolite reference resources for certain clinically important biofluids, such as cerebrospinal fluid, urine and blood. As part of an ongoing effort to systematically characterize the human metabolome through the Human Metabolome Project, we have undertaken the task of characterizing the human serum metabolome. In doing so, we have combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today's technology) in the human serum metabolome. Our use of multiple metabolomics platforms and technologies allowed us to substantially enhance the level of metabolome coverage while critically assessing the relative strengths and weaknesses of these platforms or technologies. Tables containing the complete set of 4229 confirmed and highly probable human serum compounds, their concentrations, related literature references and links to their known disease associations are freely available at http://www.serummetabolome.ca.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Myristoleic acid, ≥99% (capillary GC)
Sigma-Aldrich
L-Isoleucine, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
cis-8,11,14-Eicosatrienoic acid, ≥99%
Sigma-Aldrich
L-(+)-Lactic acid, ≥98%
Sigma-Aldrich
Phosphoric acid, BioReagent, suitable for insect cell culture, 85%
Sigma-Aldrich
cis-4,7,10,13,16,19-Docosahexaenoic acid, ≥98%
Sigma-Aldrich
L-Serine, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
L-Serine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
2-Propanol, BioReagent, for molecular biology, ≥99.5%
Sigma-Aldrich
Citric acid, anhydrous, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
Urea, meets USP testing specifications
Sigma-Aldrich
L-Asparagine, ≥98% (HPLC)
Sigma-Aldrich
L-(+)-Lactic acid, BioXtra, ≥98% (titration)
Sigma-Aldrich
Dodecanoic acid, ≥99% (GC/titration)
Sigma-Aldrich
Acetaminophen, analytical standard
Sigma-Aldrich
1,2-Dipalmitoyl-sn-glycero-3-phosphocholine, ≥99% (TLC)
Sigma-Aldrich
cis-5,8,11,14,17-Eicosapentaenoic acid, ≥99%
Sigma-Aldrich
L-Carnitine inner salt, synthetic, ≥98%
Sigma-Aldrich
Isobutyric acid, 99%
Sigma-Aldrich
Oleic acid, suitable for cell culture, BioReagent
Sigma-Aldrich
Arachidonylethanolamide, ≥97.0% (TLC), oil
Sigma-Aldrich
Oleic acid, ≥99% (GC)
Sigma-Aldrich
L-Asparagine, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
1,2-Dipalmitoyl-sn-glycero-3-phosphocholine, semisynthetic, ≥99%
Sigma-Aldrich
cis-5,8,11,14,17-Eicosapentaenoic acid, ≥85%, liquid
Sigma-Aldrich
Acetaminophen, BioXtra, ≥99.0%
Sigma-Aldrich
Creatine, anhydrous
Sigma-Aldrich
Acetaminophen, meets USP testing specifications, 98.0-102.0%, powder
Sigma-Aldrich
15(S)-Hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid, ~100 μg/mL in ethanol, ≥95%
Sigma-Aldrich
Urea, BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)