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Glucose sensors based on enzyme immobilization onto biocompatible membranes obtained by radiation-induced polymerization.

Applied biochemistry and biotechnology (1994-12-01)
L Doretti, D Ferrara, G Barison, S Lora
ABSTRACT

Amperometric glucose biosensors based on glucose oxidase immobilized onto poly(2-hydroxyethylmethacrylate) membranes obtained by gamma radiation-induced polymerization were constructed. In a three-electrode configuration, smooth or platinized platinum electrodes with different shapes were used, in order to detect the amount of hydrogen peroxide produced in the glucose oxidation. A saturated calomel electrode and a platinum foil were used as a reference and counterelectrode, respectively. The biocompatible obtained sensors were characterized as regards the temperature effect, the response, and lifetime. The determination of glucose in standard solutions was carried out, and linear calibration curves were obtained. Depending on the electrode configuration, the sensor had a response time of 1-4 min, and the measuring range extended from 5 x 10(-5) to 4 x 10(-3) M.

MATERIALS
Product Number
Brand
Product Description

Platinum, foil, light tested, 25x25mm, thickness 0.05mm, as rolled, 99.95%
Platinum, foil, 6mm disks, thickness 0.125mm, as rolled, 99.95%
Platinum, foil, 25x25mm, thickness 0.125mm, as rolled, 99.95%
Platinum, foil, 10mm disks, thickness 0.025mm, as rolled, 99.95%
Platinum, foil, not light tested, 50x50mm, thickness 0.0025mm, as rolled, 99.95%
Platinum, foil, not light tested, 50x50mm, thickness 0.002mm, as rolled, 99.95%
Platinum, foil, not light tested, 50x50mm, thickness 0.003mm, as rolled, 99.95%
Platinum, foil, not light tested, 50x50mm, thickness 0.0075mm, as rolled, 99.95%
Platinum, foil, not light tested, 50x50mm, thickness 0.0125mm, as rolled, 99.95%
Platinum, foil, not light tested, 50x50mm, thickness 0.01mm, as rolled, 99.95%
Platinum, foil, not light tested, 50x50mm, thickness 0.025mm, as rolled, 99.95%
Platinum, foil, not light tested, 50x50mm, thickness 0.025mm, as rolled, 99.99+%
Platinum, foil, 15x15mm, thickness 0.2mm, as rolled, 99.95%
Platinum, foil, 25x25mm, thickness 0.125mm, as rolled, 99.99+%
Platinum, foil, 25x25mm, thickness 0.1mm, as rolled, 99.95%
Platinum, foil, 25x25mm, thickness 0.1mm, as rolled, 99.99+%
Platinum, foil, 25x25mm, thickness 0.25mm, as rolled, 99.95%
Platinum, foil, 25x25mm, thickness 0.25mm, as rolled, 99.99+%
Platinum, foil, 25x25mm, thickness 0.25mm, as rolled, 99.999%
Platinum, foil, 30x30mm, thickness 0.2mm, as rolled, 99.95%
Platinum, foil, 50x50mm, thickness 0.125mm, as rolled, 99.95%
Platinum, foil, 50x50mm, thickness 0.125mm, as rolled, 99.99+%
Platinum, foil, 50x50mm, thickness 0.1mm, as rolled, 99.95%
Platinum, foil, 0.05m coil, thickness 0.025mm, coil width .4mm, as rolled, 99.95%
Platinum, foil, 0.05m coil, thickness 0.025mm, coil width .85mm, as rolled, 99.95%
Platinum, foil, 0.05m coil, thickness 0.025mm, coil width 1.2mm, as rolled, 99.95%
Platinum, foil, 0.05m coil, thickness 0.025mm, coil width 1.6mm, as rolled, 99.95%
Platinum, foil, 0.05m coil, thickness 0.025mm, coil width 2mm, as rolled, 99.95%
Platinum, foil, 0.05m coil, thickness 0.025mm, coil width 5mm, as rolled, 99.95%
Platinum, foil, 0.05m coil, thickness 0.025mm, coil width 8mm, as rolled, 99.95%