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Spectroscopic studies on the interaction of sodium benzoate, a food preservative, with calf thymus DNA.

Food chemistry (2013-06-19)
Guowen Zhang, Yadi Ma
ABSTRACT

The interaction between sodium benzoate (SB) and calf thymus DNA in simulated physiological buffer (pH 7.4) using acridine orange (AO) dye as a fluorescence probe, was investigated by UV-Vis absorption, fluorescence and circular dichroism (CD) spectroscopy along with DNA melting studies and viscosity measurements. An expanded UV-Vis spectral data matrix was resolved by multivariate curve resolution-alternating least squares (MCR-ALS) approach. The equilibrium concentration profiles and the pure spectra for SB, DNA and DNA-SB complex from the high overlapping composite response were simultaneously obtained. The results indicated that SB could bind to DNA, and hydrophobic interactions and hydrogen bonds played a vital role in the binding process. Moreover, SB was able to quench the fluorescence of DNA-AO complex through a static procedure. The quenching observed was indicative of an intercalative mode of interaction between SB and DNA, which was supported by melting studies, viscosity measurements and CD analysis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Deoxyribonucleic acid from calf thymus, Genomic, unsheared
Sigma-Aldrich
Deoxyribonucleic acid from calf thymus, Type XV, Activated, lyophilized powder
Sigma-Aldrich
Acridine Orange hydrochloride solution, 10 mg/mL in H2O
Sigma-Aldrich
Acridine Orange solution, 2% in H2O
Sigma-Aldrich
Sodium benzoate, BioXtra, ≥99.5%
Sigma-Aldrich
Acridine Orange base, Dye content 75 %
Sigma-Aldrich
Sodium benzoate, purum p.a., ≥99.0% (NT)
Sigma-Aldrich
Deoxyribonucleic acid, single stranded from calf thymus, lyophilized powder
Supelco
Sodium benzoate, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Sodium benzoate, Vetec, reagent grade, 98%
Sigma-Aldrich
Sodium benzoate, puriss., meets analytical specification of Ph. Eur., BP, FCC, E211, 99.0-100.5% (calc. to the dried substance), powder
Sigma-Aldrich
Acridine Orange hydrochloride hydrate, ≥98% (HPLC)