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Effect of menthol in experimentally induced ulcers: pathways of gastroprotection.

Chemico-biological interactions (2013-10-15)
A L Rozza, C A Hiruma-Lima, R K Takahira, C R Padovani, C H Pellizzon

Based on ethnopharmacological indications that Mentha species may be used in the treatment of gastrointestinal diseases, this study aimed to characterize the gastroprotective mechanisms of menthol (ME), the major compound of the essential oil from species of the genus Mentha. The gastroprotective action of ME was analyzed in gastric ulcers that were induced by ethanol or indomethacin in Wistar male rats. The mechanisms responsible for the gastroprotective effect were assessed by analyzing the amount of mucus secreted, involvement of non-protein sulfhydryl (NP-SH) compounds, involvement of calcium ion channels and NO/cGMP/K(+)ATP pathway, gastric antisecretory activity and the prostaglandin E2 (PGE2) production. The anti-diarrheal activity and acute toxicity of ME were also evaluated. Oral treatment with ME (50mg/kg) offered 88.62% and 72.62% of gastroprotection against ethanol and indomethacin, respectively. There was an increased amount of mucus and PGE2 production. The gastroprotective activity of ME involved NP-SH compounds and the stimulation of K(+)ATP channels, but not the activation of calcium ion channels or the production of NO. The oral administration of ME induced an antisecretory effect as it decreased the H(+) concentration in gastric juice. ME displayed anti-diarrheal and antiperistaltic activity. There were no signs of toxicity in the biochemical analyses performed in the rats' serum. These results demonstrated that ME provides gastroprotective and anti-diarrheal activities with no toxicity in rats.

Product Number
Product Description

Indomethacin, 98.5-100.5% (in accordance with EP)
Prostaglandin E2, synthetic, powder, BioReagent, suitable for cell culture
Menthol, 99%
Castor oil
Prostaglandin E2, ≥93% (HPLC), synthetic
(±)-Menthol, racemic, ≥98.0% (GC)
Indomethacin, Pharmaceutical Secondary Standard; Certified Reference Material
DL-Menthol, ≥95%, FCC, FG
Prostaglandin E2, γ-irradiated, powder, BioXtra, suitable for cell culture
Castor oil, tested according to Ph. Eur.
Castor oil, meets testing specifications per USP
Guanosine 3′,5′-cyclic monophosphate, ≥98% (HPLC), powder
Menthol, European Pharmacopoeia (EP) Reference Standard
Indomethacin, meets USP testing specifications
Indomethacin, European Pharmacopoeia (EP) Reference Standard
Dinoprostone, European Pharmacopoeia (EP) Reference Standard