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Serotonin metabolites in the cerebrospinal fluid in sudden infant death syndrome.

Journal of neuropathology and experimental neurology (2014-01-16)
Ingvar J Rognum, Hoa Tran, Elisabeth A Haas, Keith Hyland, David S Paterson, Robin L Haynes, Kevin G Broadbelt, Brian J Harty, Othon Mena, Henry F Krous, Hannah C Kinney
ABSTRACT

Forensic biomarkers are needed in sudden infant death syndrome (SIDS) to help identify this group among other sudden unexpected deaths in infancy. Previously, we reported multiple serotonergic (5-HT) abnormalities in nuclei of the medulla oblongata that help mediate protective responses to homeostatic stressors. As a first step toward their assessment as forensic biomarkers of medullary pathology, here we test the hypothesis that 5-HT-related measures are abnormal in the cerebrospinal fluid (CSF) of SIDS infants compared with those of autopsy controls. Levels of CSF 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA), the degradative products of 5-HT and dopamine, respectively, were measured by high-performance liquid chromatography in 52 SIDS and 29 non-SIDS autopsy cases. Tryptophan (Trp) and tyrosine (Tyr), the substrates of 5-HT and dopamine, respectively, were also measured. There were no significant differences in 5-HIAA, Trp, HVA, or Tyr levels between the SIDS and non-SIDS groups. These data preclude the use of 5-HIAA, HVA, Trp, or Tyr measurements as CSF autopsy biomarkers of 5-HT medullary pathology in infants who have died suddenly and unexpectedly. They do, however, provide important information about monoaminergic measurements in human CSF at autopsy and their developmental profile in infancy that is applicable to multiple pediatric disorders beyond SIDS.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Tryptophan, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-Tyrosine, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-Tryptophan, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%
Sigma-Aldrich
L-Tryptophan, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
L-Tyrosine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 99.0-101.0%
Supelco
Tryptophan, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-Tyrosine, BioUltra, ≥99.0% (NT)
Sigma-Aldrich
5-Hydroxyindole-3-acetic acid, ≥98% (HPLC), crystalline
Supelco
L-Tryptophan, certified reference material, TraceCERT®
Supelco
L-Tyrosine, Pharmaceutical Secondary Standard; Certified Reference Material
SAFC
L-Tyrosine
Sigma-Aldrich
Homovanillic acid, Fluorimetric reagent
Sigma-Aldrich
L-Tyrosine, FG
Supelco
L-Tyrosine, certified reference material, TraceCERT®