Nickel-dependent metalloenzymes.

Archives of biochemistry and biophysics (2013-09-17)
Jodi L Boer, Scott B Mulrooney, Robert P Hausinger
ABSTRACT

This review describes the functions, structures, and mechanisms of nine nickel-containing enzymes: glyoxalase I, acireductone dioxygenase, urease, superoxide dismutase, [NiFe]-hydrogenase, carbon monoxide dehydrogenase, acetyl-coenzyme A synthase/decarbonylase, methyl-coenzyme M reductase, and lactate racemase. These enzymes catalyze their various chemistries by using metallocenters of diverse structures, including mononuclear nickel, dinuclear nickel, nickel-iron heterodinuclear sites, more complex nickel-containing clusters, and nickel-tetrapyrroles. Selected other enzymes are active with nickel, but the physiological relevance of this metal specificity is unclear. Additional nickel-containing proteins of undefined function have been identified.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney® 2800, slurry, in H2O, active catalyst
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), Type III, powder, 15,000-50,000 units/g solid
Nickel, foam, 150x150mm, thickness 1.6mm, bulk density 0.45g/cm3, porosity 95%, 95%
Sigma-Aldrich
Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), Type IX, powder, 50,000-100,000 units/g solid
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), Type C-3, powder, ≥600,000 units/g solid
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), Type III, glycerol solution, 500-800 units/mL
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), powder, ~1 U/mg
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean)
Sigma-Aldrich
Nickel, powder, <150 μm, 99.99% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.125 mm, ≥99.9%
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), powder, white, ~8 U/mg
Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney® 2400, slurry, in H2O, active catalyst
Sigma-Aldrich
Nickel, wire, diam. 0.25 mm, ≥99.9%
Sigma-Aldrich
Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis
Sigma-Aldrich
Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis
Sigma-Aldrich
Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis
Sigma-Aldrich
Glyoxalase I from Saccharomyces cerevisiae, Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein
Nickel, mesh, 100x250mm, nominal aperture 0.73mm, wire diameter 0.25mm, 26x26 wires/inch, open area 55%, plain weave mesh, 99%
Nickel, wire reel, 20m, diameter 0.025mm, as drawn, 99.98%
Nickel, wire reel, 10m, diameter 0.01mm, as drawn, 99.98%
Nickel, rod, 100mm, diameter 16mm, 99.99+%
Nickel, foil, not light tested, 100x100mm, thickness 0.01mm, 99.95%
Nickel, wire reel, 20m, diameter 0.125mm, hard, 99.98%
Nickel, rod, 100mm, diameter 3.0mm, 99%
Nickel, wire reel, 1m, diameter 1.0mm, hard, 99.98%
Nickel, broken cathode squares, 1000g, max. size 50mm, 99.8%