Merck
  • Home
  • Search Results
  • Preservation solutions for static cold storage of abdominal allografts: which is best?

Preservation solutions for static cold storage of abdominal allografts: which is best?

Current opinion in organ transplantation (2014-02-21)
Ronald F Parsons, James V Guarrera
ABSTRACT

To update the reader on the recent literature in liver, kidney, pancreas, and intestine static cold preservation, and to identify which solutions are most advantageous for each organ. The comparison of randomized trials of histidine-tryptophan-ketoglutarate (HTK), Celsior, and University of Wisconsin solutions has shown equivalent risk of delayed graft function after kidney transplantation. Similar outcomes have been observed after pancreas preservation with University of Wisconsin, HTK, and Celsior solution. In live-donor liver transplantation, University of Wisconsin and HTK solution have shown equivalent results, whereas in the recent trials of deceased-donor liver transplantation, University of Wisconsin, HTK, and Celsior solutions have shown equivalence. Contrary to the most clinical trials, national registry data in kidney, pancreas, and liver transplantation demonstrate more detrimental effects and earlier graft loss after preservation with HTK versus University of Wisconsin solution. Early outcomes after intestinal transplantation with University of Wisconsin or HTK solution have shown no significant difference and animal studies indicate intraluminal preservation may be beneficial. The University of Wisconsin solution is the standard criterion static cold preservation for the procurement of liver, kidney, pancreas, and intestine. University of Wisconsin, HTK, and Celsior solutions all provide similar allograft outcomes in most clinical trials, but subtle differences have become more apparent in the recent studies and registry reports.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Adenosine, ≥99%
Sigma-Aldrich
Allopurinol, xanthine oxidase inhibitor
Sigma-Aldrich
Adenosine
Sigma-Aldrich
D-Mannitol, tested according to Ph. Eur.
Sigma-Aldrich
D-Mannitol, suitable for plant cell culture
Sigma-Aldrich
D-Mannitol, meets EP, FCC, USP testing specifications
Sigma-Aldrich
D-Mannitol, BioXtra, ≥98% (HPLC)
Sigma-Aldrich
D-Mannitol, ≥98%
Sigma-Aldrich
D-Mannitol, ACS reagent
Millipore
D-Mannitol, ACS reagent, suitable for microbiology, ≥99.0%
Sigma-Aldrich
D-Mannitol, BioUltra, ≥99.0% (sum of enantiomers, HPLC)
Supelco
Mannitol, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
D-Mannitol, ≥99.9999% (metals basis), for boron determination
Sigma-Aldrich
Adenosine, BioReagent, suitable for cell culture
Sigma-Aldrich
Adenosine, Vetec, reagent grade, 98%
Supelco
Allopurinol, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Adenosine, Pharmaceutical Secondary Standard; Certified Reference Material
Allopurinol, European Pharmacopoeia (EP) Reference Standard
USP
Mannitol, United States Pharmacopeia (USP) Reference Standard
Adenosine, European Pharmacopoeia (EP) Reference Standard
Mannitol, European Pharmacopoeia (EP) Reference Standard
Histidine, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Potassium chloride solution, 0.075 M, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
Potassium chloride, for molecular biology, ≥99.0%
Supelco
ISA (ionic strength adjustment solution: 1 M KCl), 1 M KCl
Sigma-Aldrich
Potassium chloride solution, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
Potassium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Supelco
Potassium chloride solution, conductance standard C acc. to ISO 7888, 0.001 M KCl
Sigma-Aldrich
Potassium chloride, tested according to Ph. Eur.
Sigma-Aldrich
L-Histidine, BioUltra, ≥99.5% (NT)