• Home
  • Search Results
  • A critical role for thymic stromal lymphopoietin in nickel-induced allergy in mice.

A critical role for thymic stromal lymphopoietin in nickel-induced allergy in mice.

Journal of immunology (Baltimore, Md. : 1950) (2014-03-29)
Meinar Nur Ashrin, Rieko Arakaki, Akiko Yamada, Tomoyuki Kondo, Mie Kurosawa, Yasusei Kudo, Megumi Watanabe, Tetsuo Ichikawa, Yoshio Hayashi, Naozumi Ishimaru
ABSTRACT

Ni is the most frequent cause of contact allergy induced by metals. However, the underlying mechanism of this induction is unknown. Our previous research demonstrates that activation of dendritic cells (DCs) through p38MAPK/MKK6 is required for Ni-induced allergy in mice. In the current study, we investigated the cellular and molecular mechanisms underlying Ni-induced allergy using a mouse model that involves injecting Ni into the ear, with or without Freund's incomplete or complete adjuvants. Nickel had greater potential to cause allergic reactions compared with palladium and gold. Among the proteins expressed at higher levels in mice with Ni-induced allergy, we focused on thymic stromal lymphopoietin (TSLP), which is produced in abundance by keratinocytes. We detected increased expression of the TSLP receptor (TSLPR) in DCs from cervical lymph nodes of mice with Ni-induced allergy, suggesting that DCs in ear tissues were activated through TSLPR signaling induced by keratinocyte-derived TSLP. Furthermore, delayed-type hypersensitivity reactions in mice with Ni-induced allergy were decreased significantly by injection of a Tslp-short interfering RNA along with atelocollagen in the ear skin. These results suggest that Ni allergy may be triggered by a TSLP/TSLPR-mediated interaction between epithelial and immune cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney® 2800, slurry, in H2O, active catalyst
Sigma-Aldrich
Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis
Sigma-Aldrich
Nickel, powder, <150 μm, 99.99% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.125 mm, ≥99.9%
Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney® 2400, slurry, in H2O, active catalyst
Sigma-Aldrich
Nickel, wire, diam. 0.25 mm, ≥99.9%
Sigma-Aldrich
Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis
Sigma-Aldrich
Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis
Sigma-Aldrich
Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis
Nickel, mesh, 100x250mm, nominal aperture 0.73mm, wire diameter 0.25mm, 26x26 wires/inch, open area 55%, plain weave mesh, 99%
Nickel, wire reel, 20m, diameter 0.025mm, as drawn, 99.98%
Nickel, wire reel, 10m, diameter 0.01mm, as drawn, 99.98%
Nickel, rod, 100mm, diameter 16mm, 99.99+%
Nickel, foil, not light tested, 100x100mm, thickness 0.01mm, 99.95%
Nickel, wire reel, 20m, diameter 0.125mm, hard, 99.98%
Nickel, rod, 100mm, diameter 3.0mm, 99%
Nickel, wire reel, 1m, diameter 1.0mm, hard, 99.98%
Nickel, broken cathode squares, 1000g, max. size 50mm, 99.8%
Nickel, tube, 1000mm, outside diameter 6.5mm, inside diameter 5.5mm, wall thickness 0.5mm, hard, 99.5%
Nickel, foil, 50mm disks, thickness 0.05mm, annealed, 99%
Nickel, wire reel, 20m, diameter 0.5mm, as drawn, 99%
Nickel, foil, 4mm disks, thickness 0.075mm, annealed, 99%
Nickel, foil, 4mm disks, thickness 0.075mm, as rolled, 99.98%
Nickel, foil, 4mm disks, thickness 0.125mm, annealed, 99%
Nickel, foil, 4mm disks, thickness 0.125mm, annealed, 99.98%
Nickel, foil, 4mm disks, thickness 0.125mm, as rolled, 100%
Nickel, foil, 4mm disks, thickness 0.125mm, as rolled, 99.99+%