Merck

Avidin-biotin technology in targeted therapy.

Expert opinion on drug delivery (2010-03-18)
Hanna P Lesch, Minna U Kaikkonen, Jere T Pikkarainen, Seppo Ylä-Herttuala
ABSTRACT

The goal of drug targeting is to increase the concentration of the drug in the vicinity of the cells responsible for disease without affecting healthy cells. Many approaches in cancer treatment are limited because of their broad range of unwanted side effects on healthy cells. Targeting can reduce side effects and increase efficacy of drugs in the patient. Avidin, originally isolated from chicken eggs, and its bacterial analogue, streptavidin, from Streptomyces avidinii, have extremely high affinity for biotin. This unique feature is the basis of avidin-biotin technology. This article reviews the current status of avidin-biotin systems and their use for pretargeted drug delivery and vector targeting. The reader will gain an understanding of the following approaches using the avidin-biotin system: i) targeting antibodies and therapeutic molecules are administered separately leading to a reduction of drug dose in normal tissues compared with conventional (radio)immunotherapies; ii) introducing avidin gene into specific tissues by local gene transfer, which subsequently can sequester and concentrate considerable amounts of therapeutic ligands; and iii) enabling transductional targeting of gene therapy vectors. Avidin and biotin technology has proved to be an extremely versatile tool with broad applications, such as pretargeting, delivering avidin gene into cells enabling targeting of biotinylated compounds and targeting of viral vectors.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Biotin, meets USP testing specifications
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Biotin, tested according to Ph. Eur.
Sigma-Aldrich
Biotin, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Biotin, ≥99.0% (T)
Supelco
Biotin, certified reference material, TraceCERT®
Sigma-Aldrich
Biotin, Vetec, reagent grade, ≥99%
Biotin, European Pharmacopoeia (EP) Reference Standard
USP
Biotin, United States Pharmacopeia (USP) Reference Standard
Supelco
Biotin, Pharmaceutical Secondary Standard; Certified Reference Material
SAFC
BIOTIN