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Streptavidin-biotin technology: improvements and innovations in chemical and biological applications.

Applied microbiology and biotechnology (2013-09-24)
Christopher M Dundas, Daniel Demonte, Sheldon Park
ABSTRACT

Streptavidin and its homologs (together referred to as streptavidin) are widely used in molecular science owing to their highly selective and stable interaction with biotin. Other factors also contribute to the popularity of the streptavidin-biotin system, including the stability of the protein and various chemical and enzymatic biotinylation methods available for use with different experimental designs. The technology has enjoyed a renaissance of a sort in recent years, as new streptavidin variants are engineered to complement native proteins and novel methods of introducing selective biotinylation are developed for in vitro and in vivo applications. There have been notable developments in the areas of catalysis, cell biology, and proteomics in addition to continued applications in the more established areas of detection, labeling and drug delivery. This review summarizes recent advances in streptavidin engineering and new applications based on the streptavidin-biotin interaction.

MATERIALS
Product Number
Brand
Product Description

Millipore
Streptavidin, 5 mg Streptavidin for ELISA & Western Blotting.
Sigma-Aldrich
Biotin, meets USP testing specifications
Sigma-Aldrich
Biotin, tested according to Ph. Eur.
Sigma-Aldrich
Biotin, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Biotin, ≥99.0% (T)
Supelco
Biotin, certified reference material, TraceCERT®
Sigma-Aldrich
Biotin, Vetec, reagent grade, ≥99%
Sigma-Aldrich
LiCl Immune Complex Wash Buffer, For use in Chromatin Immunoprecipitation assays.
Biotin, European Pharmacopoeia (EP) Reference Standard
USP
Biotin, United States Pharmacopeia (USP) Reference Standard
Supelco
Biotin, Pharmaceutical Secondary Standard; Certified Reference Material
SAFC
BIOTIN