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3'-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells.

RNA (New York, N.Y.) (2014-05-14)
Yang Eric Guo, Joan A Steitz
ABSTRACT

Synthetic 3'-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA (mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3'-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipitation assay followed by Northern blot analysis. We report that biotinylated miR-27 does not efficiently associate with Ago compared to unmodified miR-27. These results suggest that 3'-end biotin-modified miRNAs are questionable monitors of miRNA function in cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Biotin, meets USP testing specifications
Sigma-Aldrich
Biotin, tested according to Ph. Eur.
Sigma-Aldrich
Biotin, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Biotin, ≥99.0% (T)
Supelco
Biotin, certified reference material, TraceCERT®
Sigma-Aldrich
Biotin, Vetec, reagent grade, ≥99%
Biotin, European Pharmacopoeia (EP) Reference Standard
USP
Biotin, United States Pharmacopeia (USP) Reference Standard
Supelco
Biotin, Pharmaceutical Secondary Standard; Certified Reference Material
SAFC
BIOTIN