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  • ATP-binding mode including a carbamoylated lysine and two Mg(2+) ions, and substrate-binding mode in Acinetobacter baumannii MurF.

ATP-binding mode including a carbamoylated lysine and two Mg(2+) ions, and substrate-binding mode in Acinetobacter baumannii MurF.

Biochemical and biophysical research communications (2014-07-01)
Sun-Shin Cha, Young Jun An, Chang-Sook Jeong, Jeong Hee Yu, Kyung Min Chung
ABSTRACT

MurF adds d-Ala-d-Ala dipeptide to UDP-N-acetylmuramyl-l-Ala-γ-d-Glu-m-DAP (or l-Lys) in an ATP-dependent manner, which is the last step in the biosynthesis of monomeric precursor of peptidoglycan. Here we report crystal structures of two MurF-ATP complexes: the MurF-ATP complex and the MurF-ATP-UDP complex. The ATP-binding mode revealed by the crystal structure of the MurF-ATP complex confirms the previous biochemical demonstration that a carbamoylated lysine and two Mg(2+) ions are required for enzyme activity of MurF. The UDP-MurF interactions observed in the crystal structure of the MurF-ATP-UDP complex depict the characteristic substrate-binding mode of MurF. The emergence and dissemination of multidrug-resistant Acinetobacter baumannii strains are great threats to public health. Therefore, the structural information on A. baumannii MurF as a validated target for drug discovery will provide a framework to develop antibacterial agents against multidrug-resistant A. baumannii infections as well as to understand the reaction mechanism of MurF.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Lysine, ≥98% (TLC)
Sigma-Aldrich
L-Lysine monohydrochloride, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
Manganese, powder, ≥99.9% trace metals basis
Sigma-Aldrich
L-Lysine, crystallized, ≥98.0% (NT)
Supelco
L-Lysine monohydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
L-Lysine, analytical standard
Sigma-Aldrich
L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)
Sigma-Aldrich
Manganese, powder, −325 mesh, ≥99% trace metals basis
Lysine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Supelco
L-Lysine monohydrochloride, certified reference material, TraceCERT®
Sigma-Aldrich
L-Lysine acetate salt, ≥98% (HPLC)
Sigma-Aldrich
Manganese, chips, thickness <2.0 mm, 99%
Supelco
L-Lysine Acetate, Pharmaceutical Secondary Standard; Certified Reference Material
Manganese, rod, 10mm, diameter 4.0mm, cast, 99.5%
Lysine acetate, European Pharmacopoeia (EP) Reference Standard
Manganese, foil, 25x25mm, thickness 2mm, hot-pressed, 99.95%
Manganese, foil, 50x50mm, thickness 2mm, hot-pressed, 99.95%
Manganese, foil, not light tested, 100x100mm, thickness 0.0125mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 100x100mm, thickness 0.01mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 100x100mm, thickness 0.025mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 100x100mm, thickness 0.02mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.001mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.0025mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.002mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.003mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.004mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.005mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.006mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.007mm, permanent polyester support, 98.7%