Merck
  • Home
  • Search Results
  • Resveratrol modulates mitochondria dynamics in replicative senescent yeast cells.

Resveratrol modulates mitochondria dynamics in replicative senescent yeast cells.

PloS one (2014-08-08)
I-Hua Wang, Hsin-Yi Chen, Yu-Han Wang, Ko-Wei Chang, Ying-Chieh Chen, Chuang-Rung Chang
ABSTRACT

Mitochondria form a reticulum network dynamically fuse and divide in the cell. The balance between mitochondria fusion and fission is correlated to the shape, activity and integrity of these pivotal organelles. Resveratrol is a polyphenol antioxidant that can extend life span in yeast and worm. This study examined mitochondria dynamics in replicative senescent yeast cells as well as the effects of resveratrol on mitochondria fusion and fission. Collecting cells by biotin-streptavidin sorting method revealed that majority of the replicative senescent cells bear fragmented mitochondrial network, indicating mitochondria dynamics favors fission. Resveratrol treatment resulted in a reduction in the ratio of senescent yeast cells with fragmented mitochondria. The readjustment of mitochondria dynamics induced by resveratrol likely derives from altered expression profiles of fusion and fission genes. Our results demonstrate that resveratrol serves not only as an antioxidant, but also a compound that can mitigate mitochondria fragmentation in replicative senescent yeast cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Rhodamine 123, mitochondrial specific fluorescent dye
Sigma-Aldrich
Anti-HA antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-HA antibody produced in mouse, clone HA-7, ascites fluid
Sigma-Aldrich
Resveratrol, ≥99% (HPLC)
Sigma-Aldrich
Dihydroethidium, ≥95%
Sigma-Aldrich
Anti-HA antibody, Mouse monoclonal, clone HA-7, purified from hybridoma cell culture
Sigma-Aldrich
Anti-HA Tag Antibody, Upstate®, from mouse