• Home
  • Search Results
  • Deficient sucrose synthase activity in developing wood does not specifically affect cellulose biosynthesis, but causes an overall decrease in cell wall polymers.

Deficient sucrose synthase activity in developing wood does not specifically affect cellulose biosynthesis, but causes an overall decrease in cell wall polymers.

The New phytologist (2014-06-13)
Lorenz Gerber, Bo Zhang, Melissa Roach, Umut Rende, András Gorzsás, Manoj Kumar, Ingo Burgert, Totte Niittylä, Björn Sundberg
ABSTRACT

The biosynthesis of wood in aspen (Populus) depends on the metabolism of sucrose, which is the main transported form of carbon from source tissues. The largest fraction of the wood biomass is cellulose, which is synthesized from UDP-glucose. Sucrose synthase (SUS) has been proposed previously to interact directly with cellulose synthase complexes and specifically supply UDP-glucose for cellulose biosynthesis. To investigate the role of SUS in wood biosynthesis, we characterized transgenic lines of hybrid aspen with strongly reduced SUS activity in developing wood. No dramatic growth phenotypes in glasshouse-grown trees were observed, but chemical fingerprinting with pyrolysis-GC/MS, together with micromechanical analysis, showed notable changes in chemistry and ultrastructure of the wood in the transgenic lines. Wet chemical analysis showed that the dry weight percentage composition of wood polymers was not changed significantly. However, a decrease in wood density was observed and, consequently, the content of lignin, hemicellulose and cellulose was decreased per wood volume. The decrease in density was explained by a looser structure of fibre cell walls as shown by increased wall shrinkage on drying. The results show that SUS is not essential for cellulose biosynthesis, but plays a role in defining the total carbon incorporation to wood cell walls.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-(+)-Galactose, ≥99%
Sigma-Aldrich
D-Glucuronic acid, ≥98% (GC)
Sigma-Aldrich
D-(+)-Galactose, ≥98%
Sigma-Aldrich
D-(+)-Mannose, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
D-(+)-Galactose, powder, anhydrous, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
L-(−)-Fucose, ≥99%
Sigma-Aldrich
L-Rhamnose, natural sourced, 99%, FG
Sigma-Aldrich
D-(+)-Mannose, synthetic, ≥99%
Supelco
Galactose, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Inositol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
D-(−)-Arabinose, ≥98%
Sigma-Aldrich
D-(+)-Xylose, BioXtra, ≥99%
Millipore
D-(+)-Galactose, suitable for microbiology, ≥99.0%
SAFC
Galactose, plant-derived
Sigma-Aldrich
D-(+)-Mannose, wood, ≥99%
USP
Galactose, United States Pharmacopeia (USP) Reference Standard
Millipore
D-(+)-Mannose, suitable for microbiology, ≥99%
Sigma-Aldrich
D-(+)-Xylose, BioUltra, ≥99.0% (sum of enantiomers, HPLC)
Millipore
D-(−)-Arabinose, ≥99.0%, suitable for microbiology
Sigma-Aldrich
D-(+)-Galactose, BioXtra, pH 5.0-7.0 (20 °C, 1 M in H2O), ≥99%
Sigma-Aldrich
D-(+)-Mannose, BioUltra, ≥99.5% (sum of enantiomers, HPLC)
Galactose, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
D-(+)-Galactose, meets analytical specification of Ph. Eur., BP
Millipore
D-(+)-Mannose, suitable for microbiology, ≥99.0% (sum of enantiomers, HPLC)
Xylose, European Pharmacopoeia (EP) Reference Standard