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Effects of Withania somnifera on oral ethanol self-administration in rats.

Behavioural pharmacology (2014-08-15)
Alessandra T Peana, Giulia Muggironi, Liliana Spina, Michela Rosas, Sanjay B Kasture, Elisabetta Cotti, Elio Acquas
ABSTRACT

Recent evidence has shown that Withania somnifera Dunal (Ashwagandha or Indian ginseng), a herbal remedy used in traditional medicine, impairs morphine-elicited place conditioning. Here, we investigated the effect of W. somnifera roots extract (WSE) on motivation for drinking ethanol using operant self-administration paradigms. Wistar rats were trained to self-administer ethanol (10%) by nose-poking. The effects of WSE (25-75 mg/kg) were evaluated on acquisition and maintenance, on ethanol breakpoint under a progressive-ratio schedule of reinforcement and on the deprivation effect and reinstatement of seeking behaviours. Moreover, on the basis of the recent suggestion of an involvement of GABAB receptors in WSE central effects, we studied the interaction between WSE and GABAB ligands. The effect of WSE on saccharin (0.05%) oral self-administration was also tested. The results show that WSE reduced the acquisition, maintenance and breakpoint of ethanol self-administration. WSE also reduced the deprivation effect, reinstatement of ethanol-seeking behaviours and saccharin reinforcement. Furthermore, the GABAB receptor antagonist, phaclofen, counteracted the ability of WSE to impair the maintenance of ethanol self-administration. These findings show that WSE, by an action that may involve GABAB receptors, impairs motivation for drinking ethanol and suggest that further investigations should be performed to determine whether W. somnifera may represent a new approach for the management of alcohol abuse.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(±)-Baclofen, ≥98% (TLC), solid
Supelco
Mettler-Toledo Calibration substance ME 51143091, Saccharin, traceable to primary standards (LGC)
Saccharin, European Pharmacopoeia (EP) Reference Standard
USP
Saccharin, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Saccharin, ≥98%
Sigma-Aldrich
Saccharin, ≥99%
Sigma-Aldrich
Phaclofen, solid
Supelco
Saccharin, Pharmaceutical Secondary Standard; Certified Reference Material
Baclofen, European Pharmacopoeia (EP) Reference Standard