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Proteome adaptation in cell reprogramming proceeds via distinct transcriptional networks.

Nature communications (2014-12-11)
Marco Benevento, Peter D Tonge, Mira C Puri, Samer M I Hussein, Nicole Cloonan, David L Wood, Sean M Grimmond, Andras Nagy, Javier Munoz, Albert J R Heck
ABSTRACT

The ectopic expression of Oct4, Klf4, c-Myc and Sox2 (OKMS) transcription factors allows reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). The reprogramming process, which involves a complex network of molecular events, is not yet fully characterized. Here we perform a quantitative mass spectrometry-based analysis to probe in-depth dynamic proteome changes during somatic cell reprogramming. Our data reveal defined waves of proteome resetting, with the first wave occurring 48 h after the activation of the reprogramming transgenes and involving specific biological processes linked to the c-Myc transcriptional network. A second wave of proteome reorganization occurs in a later stage of reprogramming, where we characterize the proteome of two distinct pluripotent cellular populations. In addition, the overlay of our proteome resource with parallel generated -omics data is explored to identify post-transcriptionally regulated proteins involved in key steps during reprogramming.

MATERIALS
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Product Description

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