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Protective effects of hesperidin on concanavalin A-induced hepatic injury in mice.

International immunopharmacology (2014-05-29)
Gang Li, Mao-Jian Chen, Chao Wang, Hao Nie, Wen-Jian Huang, Ting-Dong Yuan, Ting Sun, Ke-Gang Shu, Chang-Fu Wang, Quan Gong, Shao-Qian Tang
ABSTRACT

Hesperidin (HDN) is a citrus bioflavonoid, which widely exists in many plants. Previous researches have proved that HDN has several functions such as anti-oxidant, anti-tumor, anti-inflammatory, immune regulation and so on. In the present study, we explored the protective effects of HDN on concanavalin A (Con A)-induced hepatic injury. Acute hepatic injury model was established successfully by intravenous administration of Con A (15 mg/kg) in male C57BL/6 mice, and HDN was pretreated for 10 days before Con A challenge. It was found that the hepatic injury was notably improved in HDN pretreated mice. Furthermore, hepatic oxidative stress and the production of proinflammatory cytokines including TNF-α and IFN-γ were decreased by HDN pretreatment. More importantly, compared with Con A-treated mice, the expression and releasing of HMGB1 and T-cell activation were markedly reduced in HDN pretreated mice. Thus, these results suggest that HDN protects mice from Con A-induced hepatic injury by suppressing hepatocyte oxidative stress, producing cytokines, expressing and releasing HMGB1 and activating T cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-HMGB1 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-HMGB1 (AB2) antibody produced in rabbit, IgG fraction of antiserum