Merck
  • Home
  • Search Results
  • Effect of 1,3-propanediol, organic acids, and ethanol on growth and metabolism of Clostridium butyricum DSP1.

Effect of 1,3-propanediol, organic acids, and ethanol on growth and metabolism of Clostridium butyricum DSP1.

Applied microbiology and biotechnology (2014-12-20)
Daria Szymanowska-Powałowska, Piotr Kubiak
ABSTRACT

Knowledge of tolerance of bacteria to toxic stress is important, especially for processes targeted at high final titers of product. Information on environmental limits and stress responses may help during selection of strains or design and control of processes. The influence of the main product and its co-products on the process of 1,3-propanediol (PD) synthesis was determined. Adaptation to toxic compounds was noticed as Clostridium butyricum DSP1 was less sensitive to the addition of these factors during its exponential growth on glycerol than when the factor was present in the medium before inoculation. It was also shown that the response of the tested strain to the toxicity of 1,3-propanediol (1,3-PD) has different proteomic profiles depending on the stage of culture when this substance is introduced. Relatively satisfactory activity of the analyzed strain was sustained up to a concentration of 1,3-PD of 40 g/L while 80 g/L of this metabolite was lethal to the bacterium. As for the by-products, acetic acid was determined to be the most toxic among the acids excreted during the process.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glycerol, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
Sigma-Aldrich
Glycerol, for molecular biology, ≥99.0%
Sigma-Aldrich
DL-Lactic acid, 85 % (w/w), syrup
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Sigma-Aldrich
Butyric acid, ≥99%, FG
Sigma-Aldrich
Butyric acid, ≥99%
Sigma-Aldrich
Glycerol, BioXtra, ≥99% (GC)
Sigma-Aldrich
Glycerol, ≥99.5%
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
1,3-Propanediol, 98%
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
Butyric acid, natural, ≥99%, FCC, FG
Sigma-Aldrich
Glycerol, FCC, FG
Sigma-Aldrich
Lactic acid solution, ACS reagent, ≥85%
Supelco
Acetonitrile, analytical standard
Sigma-Aldrich
Acetonitrile, puriss. p.a., ACS reagent, ≥99.5% (GC)
Sigma-Aldrich
DL-Lactic acid, ~90% (T)
Supelco
Butyric acid, analytical standard
Sigma-Aldrich
Glycerol, BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)
Sigma-Aldrich
Glycerol, tested according to Ph. Eur., anhydrous
Millipore
Bifido Selective Supplement B, suitable for microbiology
Sigma-Aldrich
Acetonitrile, Preparateur, ≥99.9% (GC), One-time steel-plastic (SP) drum
Sigma-Aldrich
Acetonitrile, for DNA synthesis
Supelco
5α-Androstan-17β-ol-3-one, VETRANAL®, analytical standard
Sigma-Aldrich
Acetonitrile, Preparateur, ≥99.9% (GC), Customized plastic drum
Sigma-Aldrich
Ultrapure Acetonitrile
Supelco
Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Glycerol, Vetec, reagent grade, 99%
USP
Glacial acetic acid, United States Pharmacopeia (USP) Reference Standard