Merck

New cholinesterase inhibitors from Garcinia atroviridis.

Fitoterapia (2014-06-14)
Wen-Nee Tan, Melati Khairuddean, Keng-Chong Wong, Kooi-Yeong Khaw, Murugaiyah Vikneswaran
ABSTRACT

A triflavanone, Garcineflavanone A (1) and a biflavonol, Garcineflavonol A (2) have been isolated from the stem bark of Garcinia atroviridis (Clusiaceae), collected in Peninsular Malaysia. Their structures were established using one and two-dimensional NMR, UV, IR and mass spectrometry and evaluated in vitro for their acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes inhibitory activity. Molecular docking studies of the isolated compounds were performed using docking procedure of AutoDock to disclose the binding interaction and orientation of these molecules into the active site gorge.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
5,5′-Dithiobis(2-nitrobenzoic acid), ReagentPlus®, 99%
Sigma-Aldrich
5,5′-Dithiobis(2-nitrobenzoic acid), ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Sigma-Aldrich
S-Butyrylthiocholine chloride, crystalline
Supelco
Phosphate Standard for IC, TraceCERT®, 1000 mg/L phosphate in water
Sigma-Aldrich
Sodium phosphate dibasic solution, BioUltra, 0.5 M in H2O
Sigma-Aldrich
Sodium phosphate monobasic-16O4, 99.9 atom % 16O
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5,5′-Dithiobis(2-nitrobenzoic acid), Vetec, reagent grade, 98%
Sigma-Aldrich
Sodium phosphate dibasic, BioXtra, ≥99.0%
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Sodium phosphate monobasic, ReagentPlus®, ≥99.0%
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Sodium phosphate dibasic, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%
Sigma-Aldrich
Sodium phosphate dibasic, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Sodium phosphate dibasic, for molecular biology, ≥98.5% (titration)
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Sodium phosphate monobasic, BioReagent, for molecular biology, anhydrous, ≥98%
Sigma-Aldrich
Sodium phosphate monobasic, meets USP testing specifications, anhydrous
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Sodium phosphate monobasic, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0% (titration)
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Acetylthiocholine iodide, ≥98% (TLC), powder or crystals
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Sodium phosphate monobasic, BioXtra, ≥99.0%
Sigma-Aldrich
Sodium phosphate monobasic, 99.998% trace metals basis
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Sodium phosphate monobasic, purum p.a., anhydrous, ≥99.0% (T)
Sigma-Aldrich
Sodium phosphate dibasic, 99.95% trace metals basis
Sigma-Aldrich
Acetylthiocholine iodide, ≥99.0% (AT)
Sigma-Aldrich
Sodium phosphate dibasic, BioUltra, for molecular biology, ≥99.5% (T)
Sigma-Aldrich
Sodium phosphate dibasic, purum p.a., anhydrous, ≥98.0% (T)
Sigma-Aldrich
Sodium phosphate monobasic solution, BioUltra, 5 M in H2O
Sigma-Aldrich
Sodium phosphate dibasic, Vetec, reagent grade, 99%
Sigma-Aldrich
Sodium phosphate monobasic, Vetec, reagent grade, 99%
Sigma-Aldrich
Sodium phosphate dibasic, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%, free-flowing, Redi-Dri
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Sodium phosphate dibasic, puriss. p.a., ACS reagent, anhydrous, ≥99.0% (T)
Sigma-Aldrich
Sodium phosphate dibasic, ACS reagent, ≥99.0%
Sigma-Aldrich
Sodium phosphate monobasic, anhydrous, free-flowing, Redi-Dri, ≥99.0%