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  • Topical rapamycin systematically suppresses the early stages of pulsed dye laser-induced angiogenesis pathways.

Topical rapamycin systematically suppresses the early stages of pulsed dye laser-induced angiogenesis pathways.

Lasers in surgery and medicine (2014-10-02)
Lin Gao, Sydney Phan, Dawnica Mercado Nadora, Margarita Chernova, Victor Sun, Salena Marie Oaxaca Preciado, Brittany Ballew, Zhenyu Jia, Wangcun Jia, Gang Wang, Martin C Mihm, J Stuart Nelson, Wenbin Tan
ABSTRACT

Administration of topical rapamycin (RPM) suppresses the regeneration and revascularization of photocoagulated blood vessels induced by pulsed dye laser (PDL). To systematically elucidate the molecular pathophysiology of the inhibition of PDL-induced angiogenesis by topical RPM in a rodent model. The mRNA expression profiles of 86 angiogenic genes and phosphorylation levels of ribosomal protein S6 kinase (P70S6K) in rodent skin were examined with or without topical RPM administration post-PDL exposure. The PDL-induced systematic increases in transcriptional levels of angiogenic genes showed a peak expression at days 3-7 post-PDL in rodent skin. Topical application of 1% RPM significantly and systematically suppressed the PDL-induced increase in mRNA levels of the examined angiogenic genes during the first five days post-PDL. The phosphorylation levels of P70S6K increased after PDL exposure but those increases were suppressed by the topical RPM. After topical application, RPM penetrated to an approximate depth of 768.4 μm into rodent skin. Topical application of 1% RPM can significantly and systematically suppress the PDL-induced early stage of angiogenesis via inhibition of the AKT/mTOR/P70S6K pathway in a rodent model.

MATERIALS
Product Number
Brand
Product Description

Supelco
Formaldehyde solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
Sigma-Aldrich
Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Sigma-Aldrich
Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
Sigma-Aldrich
Formaldehyde solution, tested according to Ph. Eur.
Sigma-Aldrich
Formaldehyde solution, meets analytical specification of USP, ≥34.5 wt. %
Sigma-Aldrich
Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
Sigma-Aldrich
Formaldehyde solution, for molecular biology, 36.5-38% in H2O
Sigma-Aldrich
Formaldehyde solution, meets analytical specification of Ph. Eur., BP, 35 wt. %, contains 10% methanol as stabilizer
SAFC
Formaldehyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H2O