Analysis of eight glycols in serum using LC-ESI-MS-MS.

Journal of analytical toxicology (2014-09-05)
Laurent Imbert, Elodie Saussereau, Christian Lacroix
ABSTRACT

A liquid chromatography coupled with electrospray tandem mass spectrometry method was developed for the analysis of ethylene glycol, diethylene glycol, triethylene glycol, 1,4-butanediol, 1,2-butanediol, 2,3-butanediol, 1,2-propanediol and 1,3-propanediol, in serum after a Schotten-Baumann derivatization by benzoyl chloride. Usual validation parameters were tested: linearity, repeatability and intermediate precision, limits of detection and quantification, carry over and ion suppression. Limits of detection were between 0.18 and 1.1 mg/L, and limits of quantification were between 0.4 and 2.3 mg/L. Separation of isomers was possible either chromatographically or by selecting specific multiple reaction monitoring transitions. This method could be a useful tool in case of suspected intoxication with antifreeze agents, solvents, dietary supplements or some medical drug compounds.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
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Methanol, anhydrous, 99.8%
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Methanol, HPLC Plus, ≥99.9%
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Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
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Methanol, suitable for HPLC, gradient grade, ≥99.9%
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Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
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Acetonitrile, anhydrous, 99.8%
Supelco
Methanol, analytical standard
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Sodium hydroxide, BioXtra, ≥98% (acidimetric), pellets (anhydrous)
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Ethylene glycol, anhydrous, 99.8%
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Sodium hydroxide, ACS reagent, ≥97.0%, pellets
Supelco
Ammonium formate, eluent additive for LC-MS, LiChropur, ≥99.0%
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Formic acid, reagent grade, ≥95%
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Sodium hydroxide solution, 50% in H2O
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Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
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Acetonitrile, HPLC Plus, ≥99.9%
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Sodium hydroxide, reagent grade, ≥98%, pellets (anhydrous)
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Methanol, ACS reagent, ≥99.8%
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Ethylene glycol, ReagentPlus®, ≥99%
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Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H2O
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Sodium hydroxide solution, 1.0 N, BioReagent, suitable for cell culture
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Formic acid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
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Pentane, anhydrous, ≥99%
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1,4-Butanediol, ReagentPlus®, 99%
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Propylene Glycol, meets USP testing specifications
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Formic acid, ACS reagent, ≥96%
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Propylene glycol, FCC, FG
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Diethylene glycol, ReagentPlus®, 99%
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1,2-Propanediol, ACS reagent, ≥99.5%