Merck
  • Home
  • Search Results
  • Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation.

Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation.

Pharmaceutical biology (2014-12-05)
Rayhana Begum, Manju Sharma, K K Pillai, Vidhu Aeri, Manjur Ali Sheliya
ABSTRACT

Careya arborea Roxb. (Lecythidaceae) has multiple applications in traditional medicine; it exhibits analgesic, antibacterial, anti-inflammatory, antiulcer, and protective effects. However, the effect of C. arborea on biochemical and immmunological inflammatory mediators has not been explored. The present study investigates the anti-inflammatory potential of the methanol extract of C. arborea stem bark and further assesses its possible mechanism on the modulation of inflammatory biomarkers. Anti-inflammatory activity of C. arborea methanol extract (CAME) was evaluated (100 and 200 mg/kg, p.o) using indomethacin (10 mg/kg, p.o) as the standard drug in Wistar albino rats. Inflammation was induced by injecting 0.1 ml carrageenan (1% w/v) into the left hind paw. The anti-inflammatory mechanism was studied by measuring malondialdehyde (MDA), C-reactive protein (CRP), nitric oxide (NO), myeloperoxidase (MPO), TNF-α, and IL-1β levels in both control and treated groups. A protocol has also been established to quantify quercetin and betulinic acid content in CAME using HPTLC fingerprint. Careya arborea significantly (p < 0.001) decreased carrageenan-induced paw edema, showed a reduction of 48.87 and 65.53% at doses of 100 and 200 mg/kg, respectively. Moreover, CAME significantly decreased the MDA, CRP, NO, and MPO levels, elevated by carrageenan induced inflammation. CAME also markedly down-regulated serum TNF-α and IL-1β levels. These findings were further supported by the histological study. The content of quercetin and betulinic acid in CAME was found to be 0.177 and 3.14%, respectively. Several mechanisms, including the inhibition of pro-inflammatory cytokines, enzymes and mediators release, appear to account for the anti-inflammatory potential of C. arborea.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trichloroacetic acid solution, 6.1 N (approx. 100% w/v)
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥98%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioXtra, ≥99%
Sigma-Aldrich
Trichloroacetic acid solution, 6.1 N
Supelco
o-Dianisidine, for spectrophotometric det. of Au, NO2-, Ce(IV), for the detection of Au, Co, Cu, SCN-, V, ≥97.0%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)
Supelco
Hexadecyltrimethylammonium bromide, suitable for ion pair chromatography, LiChropur
Supelco
Hexadecyltrimethylammonium bromide, analytical standard
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥96.0% (AT)
Sigma-Aldrich
o-Dianisidine, peroxidase substrate
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, Vetec, reagent grade, 96%
SAFC
Hexadecyltrimethylammonium bromide
Supelco
Sulfanilamide melting point standard, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Quercetin, ≥95% (HPLC), solid
Supelco
Betulinic acid, analytical standard
USP
Sulfanilamide Melting Point Standard, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
2-Thiobarbituric acid, ≥98%
Sigma-Aldrich
Indomethacin, meets USP testing specifications
Sigma-Aldrich
Trichloroacetic acid, BioXtra, ≥99.0%
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, ≥99.0%
Sigma-Aldrich
Betulinic acid, ≥98% (HPLC)
Sigma-Aldrich
Indomethacin, 98.5-100.5% (in accordance with EP)
Sigma-Aldrich
Trichloroacetic acid, ≥99.0% (titration)
Sigma-Aldrich
Trichloroacetic acid, suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%
Sigma-Aldrich
Hydrogen peroxide solution, tested according to Ph. Eur.
Sigma-Aldrich
Trichloroacetic acid, puriss., meets analytical specification of Ph. Eur., USP 21, 99-100.5% (calc. to the dried substance)
Sigma-Aldrich
Betulinic acid, technical grade, 90%
Sigma-Aldrich
Trichloroacetic acid, BioUltra, ≥99.5% (T)
Sigma-Aldrich
Trichloroacetic acid, purum p.a., ≥99.0% (T)