In this work, ultra high performance liquid chromatography was used for the characterization of non polar lipids (triacylglycerols) in milk samples of different origin, as well as milk-derivatives. For tackling such a task, three core-shell type octadecylsilica columns were serially coupled, reaching a total stationary phase length of 45cm, using acetonitrile-isopropanol gradient elution allowing triacylglicerol separation according to increasing partition number. The employment of an ion-trap-time-of-flight detection in conjunction with atmospheric-pressure chemical ionization mass spectrometry was carried out to positively identify a number of 243 different triacylglycerols containing up to 22 fatty acids, with 2-22 carbon atom alkyl chain length, and 0-3 double bonds. This work reports an extensive characterization of the triacylglycerol fraction in milk and milk-related samples of different sources.
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