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Liquid Chromatographic Analysis of the Interaction between Amino Acids and Aromatic Surfaces Using Single-Wall Carbon Nanotubes.

Langmuir : the ACS journal of surfaces and colloids (2015-07-25)
Kazuki Iwashita, Kentaro Shiraki, Rieko Ishii, Takeshi Tanaka, Atsushi Hirano
ABSTRACT

Proteins have nonspecific adsorption capacities for solid surfaces. Although the nonspecific adsorption capacities are generally understood to be related to the hydrophobicity or charge density of the surfaces, little is known at the amino acid level about the interaction between proteins and polyaromatic surfaces such as carbon nanotubes, which have recently been used for biotechnology applications. In this study, we investigated the interaction between proteinogenic amino acids and carbon nanotubes using high-performance liquid chromatography on silica matrices coated by single-wall carbon nanotubes (SWCNTs). Among the amino acids used in this study, tryptophan, tyrosine, and phenylalanine showed exceptional affinity for the matrices. The characteristic affinities of these amino acids were ascribed to their unique interactions with the large polyaromatic surfaces of the SWCNTs. These results are useful for understanding and controlling protein adsorption onto aromatic surfaces.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Phenylalanine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-Phenylalanine, reagent grade, ≥98%
Sigma-Aldrich
L-Isoleucine, BioUltra, ≥99.5% (NT)
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Urea, BioUltra, for molecular biology, ≥99% (T)
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Citric acid, BioUltra, anhydrous, ≥99.5% (T)
Sigma-Aldrich
Sodium deoxycholate, BioXtra, ≥98.0% (dry matter, NT)
Sigma-Aldrich
Urea, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%
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Citric acid, ≥99.5%, FCC, FG
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Ammonium-14N2 sulfate, 99.99 atom % 14N
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Ammonium sulfate, for molecular biology, ≥99.0%
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L-Phenylalanine, 99%
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L-Alanine-12C3, 99.9 atom % 12C
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L-Phenylalanine, 99%, FCC
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Ammonium sulfate, 99.999% trace metals basis
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2-Hydroxybutyric acid sodium salt, 97%
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Citric acid, ACS reagent, ≥99.5%
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Ammonium sulfate, BioUltra, ≥99.0% (T)
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Urea, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)
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L-Tryptophan, BioUltra, ≥99.5% (NT)
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L-Phenylalanine, BioUltra, ≥99.0% (NT)
SAFC
L-Isoleucine
SAFC
L-Phenylalanine
Sigma-Aldrich
DL-Tyrosine, 99%
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Sodium deoxycholate, ≥97% (titration)
Sigma-Aldrich
L-Isoleucine, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-Isoleucine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
Ammonium sulfate, BioXtra, ≥99.0%
Sigma-Aldrich
Ammonium sulfate, suitable for plant cell culture, ≥99.0%
Sigma-Aldrich
L-Tryptophan, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%
Sigma-Aldrich
L-Tryptophan, reagent grade, ≥98% (HPLC)