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Functional Reconstruction of Tracheal Defects by Protein-Loaded, Cell-Seeded, Fibrous Constructs in Rabbits.

Tissue engineering. Part A (2015-06-23)
Lindsey M Ott, Cindy H Vu, Ashley L Farris, Katrina D Fox, Richard A Galbraith, Mark L Weiss, Robert A Weatherly, Michael S Detamore
ABSTRACT

Tracheal stenosis is a life-threatening disease and current treatments include surgical reconstruction with autologous rib cartilage and the highly complex slide tracheoplasty surgical technique. We propose using a sustainable implant, composed of a tunable, fibrous scaffold with encapsulated chondrogenic growth factor (transforming growth factor-beta3 [TGF-β3]) or seeded allogeneic rabbit bone marrow mesenchymal stromal cells (BMSCs). In vivo functionality of these constructs was determined by implanting them in induced tracheal defects in rabbits for 6 or 12 weeks. The scaffolds maintained functional airways in a majority of the cases, with the BMSC-seeded group having an improved survival rate and the Scaffold-only group having a higher occurrence of more patent airways as determined by microcomputed tomography. The BMSC group had a greater accumulation of inflammatory cells over the graft, while also exhibiting normal epithelium, subepithelium, and cartilage formation. Overall, it was concluded that a simple, acellular scaffold is a viable option for tracheal tissue engineering, with the intraoperative addition of cells being an optional variation to the scaffolds.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acid Fuchsin, used in tissue staining
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2-Hydroxybutyric acid sodium salt, 97%
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Picric acid, moistened with water, ≥98%
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