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Rapid prototyping of microfluidic chips for dead-volume-free MS coupling.

Analytical and bioanalytical chemistry (2015-09-24)
Claudia Dietze, Tobias Scholl, Stefan Ohla, Johannes Appun, Christoph Schneider, Detlev Belder
ABSTRACT

A fast and straightforward method to prototype microfluidic chip systems for dead-volume-free hyphenation to electrospray-ionisation mass spectrometry is presented. The developed approach based on liquid-phase lithography provides an inexpensive and reliable access to microfluidic chips for MS coupling which can be manufactured in any laboratory with low technical demands. The rapid prototyping approach enables the seamless integration of capillaries serving as electrospray emitters with negligible dead volume. The high versatility of the presented prototyping method and the applicability of a variety of chip-based devices in different fields of lab-on-a-chip technology are established for analytical separations by means of chip-electrochromatography-MS and for continuous-flow synthesis using microreactor technology with MS detection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
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2-Propanol, BioReagent, for molecular biology, ≥99.5%
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
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Acetic acid, glacial, ReagentPlus®, ≥99%
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2-Propanol, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetic acid, glacial, ACS reagent, ≥99.7%
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
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2-Propanol, anhydrous, 99.5%
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Sodium phosphate monobasic, ReagentPlus®, ≥99.0%
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Ammonia solution, 7 N in methanol
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Isopropyl alcohol, ≥99.7%, FCC, FG
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Acetonitrile, ACS reagent, ≥99.5%
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Ammonium acetate, for molecular biology, ≥98%
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Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
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Heptane, anhydrous, 99%
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Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
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Heptane, ReagentPlus®, 99%
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Isopropyl alcohol, meets USP testing specifications
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Ammonium acetate, reagent grade, ≥98%
Sigma-Aldrich
Ammonia, anhydrous, ≥99.98%
Sigma-Aldrich
2-Propanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
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Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
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2-Propanol, BioUltra, for molecular biology, ≥99.5% (GC)
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Ammonium acetate, ACS reagent, ≥97%
Sigma-Aldrich
Acetic acid, glacial, ≥99.99% trace metals basis
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Sodium phosphate monobasic, BioReagent, for molecular biology, anhydrous, ≥98%
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Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
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Ammonium acetate solution, for molecular biology, 7.5 M
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Acetic acid, natural, ≥99.5%, FG