• Home
  • Search Results
  • Characterization of the in vivo host response to a bi-labeled chitosan-dextran based hydrogel for postsurgical adhesion prevention.

Characterization of the in vivo host response to a bi-labeled chitosan-dextran based hydrogel for postsurgical adhesion prevention.

Journal of biomedical materials research. Part A (2014-12-30)
Jaydee D Cabral, Michelle A McConnell, Clare Fitzpatrick, Sonya Mros, Gail Williams, Peter J Wormald, Stephen C Moratti, Lyall R Hanton
ABSTRACT

In developing a chitosan/dextran-based (CD) hydrogel as an adhesion prevention postsurgical aid, the in vivo biodegradation rate, biodistribution, and inflammatory response are important parameters to the biomedical device design. Herein, for the first time, a CD hydrogel was prepared by mixing aqueous solutions of a near infrared (NIR) labeled succinylated chitosan (SC) and tritiated [(3) H] oxidized dextran (DA). The biodegradation and biodistribution of the NIR/[(3) H]-CD hydrogel was tracked noninvasively using NIR fluorescence imaging, and by liquid scintillation counting (LSC) of organs/tissues after subcutaneous injection in BALB/c mice. The inflammatory response was assessed by measuring serum cytokine levels using a Bio-plex assay and by histological examination of injection site tissue. Fluorescence imaging showed the hydrogel to degrade in under a week. LSC revealed the hydrogel to reside mainly at the injection site, and excreted primarily via the urine within the first 48 h. The CD hydrogel showed a mild inflammatory response as cytokine levels were comparable to saline injected controls. Histological examination of injection site tissue confirmed the cytokine results. In summary, the CD hydrogel's in vivo biodegradation rate, biodistribution, and inflammatory response was determined. Our results indicate that the CD hydrogel has an appropriate biocompatibility after s.c. administration.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium borohydride, powder, ≥98.0%
Sigma-Aldrich
Sodium borohydride, ReagentPlus®, 99%
Sigma-Aldrich
Sodium borohydride, purum p.a., ≥96% (gas-volumetric)
Sigma-Aldrich
Sodium borohydride, granular, 99.99% trace metals basis
Sigma-Aldrich
Sodium borohydride solution, ~12 wt. % in 14 M NaOH
Sigma-Aldrich
Sodium borohydride, granular, 10-40 mesh, 98%
Sigma-Aldrich
Sodium borohydride solution, 2.0 M in triethylene glycol dimethyl ether
Sigma-Aldrich
VenPure® SF, powder
Sigma-Aldrich
Sodium borohydride, caplets (18 × 10 × 8 mm), 98%
Sigma-Aldrich
Sodium borohydride, 90% NaBH4 basis, pellets, diam. 8 mm, Cobalt (Doped)
Sigma-Aldrich
Sodium borohydride solution, 0.5 M in 2-methoxyethyl ether
Sigma-Aldrich
Sodium borohydride, hydrogen-storage grade, 98%