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Comparison of retention properties of stationary phases imitated cell membrane in RP HPLC.

Comparison of retention properties of stationary phases imitated cell membrane in RP HPLC.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2015-04-23)

Szymon Bocian, Bogusław Buszewski

ABSTRACT

Chromatographic properties of two columns was compared: commercial IAM.PC.DD2 that imitates the cell membrane and home-made Amino-P-C18 (N,O-dialkylphosphoramidate C18). The comparison has been done by correlation of retention (logkw parameters) of a series of solutes: hydrophobic (alkyl benzene derivatives and PAHs) and polar, with both acidic (flavonoids) and basic (nucleosides and nucleic bases) character. The slope of correlation plots for hydrophobic compounds and polar basic was very close to 1.0 that confirms the chromatographic similarity. Only for flavonoids the slope of correlation plot was 1.5. For hydrophobic compound retention parameters logkw were also correlated with hydrophobic parameter logP with very good determination coefficients.

MATERIALS

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