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Impact of DNA polymerases and their buffer systems on quantitative real-time PCR.

Journal of clinical microbiology (2004-01-13)
Petra Wolffs, Halfdan Grage, Oskar Hagberg, Peter Rådström
ABSTRACT

An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

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Roche
Tth DNA Polymerase, pkg of 500 U (2 x 250_U), sufficient for ≤200 reactions