Versatile wetting measurement of microplate wells.

The Review of scientific instruments (2016-12-03)
Enoch Ming Wei Ng, Brandon Huey-Ping Cheong, Yang Yu, Oi Wah Liew, Tuck Wah Ng

A method to measure the contact angle, which is indicative of wetting, using small liquid volumes dispensed directly on microplate wells is described and demonstrated. Experiments with enhanced green protein samples of volumes 4.4-6 μl showed no measured variance in the contact angle. Experiments with phosphate buffer solution with varied concentrations of a non-ionic detergent (Tween 20) dissolved, however, revealed smaller contact angles with increased detergent concentration. It is experimentally shown that drops can be located up to 7° from the lowest position of the well without affecting the accuracy of contact angle measurements. Numerical simulations confirm the ability of the drops to manifest the correct contact angle despite the lack of axis-symmetry in their shape while residing on a circular surface. This method offers a convenient means to determine the wetting characteristics of different liquid samples in different microplates.

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Product Description

TWEEN® 20, viscous liquid, suitable for cell culture